Fig. 3. HOTAIR knockdown decreases the activity Wnt/β-catenin signaling pathway in HeLa cells. HeLa cells were transiently transfected with DsiCTRL or DsiHOTAIR and 500 ng of FOPFlash-Luc (mutant reporter vector) or 500 ng of TOPFlash-Luc (canonical Wnt reporter vector) plasmids and the luciferase activity was determined after 48 (A). mRNA level of SNAIL, EDN3, CYCD1 and SPRY2 was determined by RT-qPCR (B). Representative inmunoblots of β-catenin expression in cytoplasm and nucleus. Tubulin and lamin β were used as loading controls, cytoplasmic and nuclear respectively, below is shown the densitometry analysis (C). The bars represent the mean ± standard deviation from at least three independent experiments. *p˂0.05.